Engineering of next generation cyber-physical automation system architectures

Cyber-Physical-Systems (CPS) enable flexible and reconfigurable realization of automation system architectures, utilizing distributed control architectures with non-hierarchical modules linked together through different communication systems. Several control system architectures have been developed and validated in the past years by research groups. However, there is still a lack of implementation in industry. The intention of this work is to provide a summary of current alternative control system architectures that could be applied in industrial automation domain as well as a review of their commonalities. The aim is to point out the differences between the traditional centralized and hierarchical architectures to discussed ones, which rely on decentralized decision-making and control. Challenges and impacts that industries and engineers face in the process of adopting decentralized control architectures are discussed, analysing the obstacles for industrial acceptance and the new necessary interdisciplinary engineering skills. Finally, an outlook of possible mitigation and migration actions required to implement the decentralized control architectures is addressed.The authors would like to thank the European Commission for the support, and the partners of the EU Horizon 2020 project PERFoRM (2016b) for the fruitful discussions. The PERFoRM project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 680435.info:eu-repo/semantics/publishedVersio

Towards the integration of process and quality control using multi-agent technology

The paper introduces a vision on the design of distributed manufacturing control systems using the multi-agent principles to enhance the integration of the production and quality control processes. It is highlighted how agent technology may enforce interaction of manufacturing execution system and distributed control system, enhancing the exploitation of the available information at the quality control and process control levels. A specific focus is made on a suitable engineering methodology for the design and realization of such concept. Innovation is also presented at the level of adaptive process control and self-optimizing quality control, with examples related to a home appliance production line

Instantiating the PERFORM system architecture for industrial case studies

The PERFoRM project, an innovation action promoted within the scope of the EU Horizon 2020 program, advocates the use of an Industrie 4.0 compliant system architecture for the seamless reconfiguration of robots and machinery. The system architecture re-uses the innovative results from previous successful R & D projects on distributed control systems domain, such as SOCRADES, IMC-AESOP, GRACE and IDEAS. This paper, after describing the main pillars of the PERFoRM system architecture, focuses on mapping the system architecture into four industrial use cases aiming to validate the system architecture design before its deployment in the real environments.info:eu-repo/semantics/publishedVersio

Viable adhered Staphylococcus aureus highly reduced on novel antimicrobial sutures using chlorhexidine and octenidine to avoid surgical site infection (SSI)

Background Surgical sutures can promote migration of bacteria and thus start infections. Antiseptic coating of sutures may inhibit proliferation of adhered bacteria and avoid such complications. Objectives This study investigated the inhibition of viable adhering bacteria on novel antimicrobially coated surgical sutures using chlorhexidine or octenidine, a critical factor for proliferation at the onset of local infections. The medical need, a rapid eradication of bacteria in wounds, can be fulfilled by a high antimicrobial efficacy during the first days after wound closure. Methods As a pretesting on antibacterial efficacy against relevant bacterial pathogens a zone of inhibition assay was conducted with middle ranged concentrated suture coatings (22 mu g/cm). For further investigation of adhering bacteria in detail the most clinically relevant Staphylococcus aureus (ATCC (R) 49230 (TM)) was used. Absorbable braided sutures were coated with chlorhexidine-laurate, chlorhexidine-palmitate, octenidine-laurate, and octenidine-palmitate. Each coating type resulted in 11, 22, or 33 mu g/cm drug content on sutures. Scanning electron microscopy (SEM) was performed once to inspect the coating quality and twice to investigate if bacteria have colonized on sutures. Adhesion experiments were assessed by exposing coated sutures to S. aureus suspensions for 3 h at 37 degrees C. Subsequently, sutures were sonicated and the number of viable bacteria released from the suture surface was determined. Furthermore, the number of viable planktonic bacteria was measured in suspensions containing antimicrobial sutures. Commercially available sutures without drugs (Vicryl (R), PGA Resorba (R), and Gunze PGA), as well as triclosan-containing Vicryl (R) Plus were used as control groups. Results Zone of inhibition assay documented a multispecies efficacy of novel coated sutures against tested bacterial strains, comparable to most relevant S. aureus over 48 hours. SEM pictures demonstrated uniform layers on coated sutures with higher roughness for palmitate coatings and sustaining integrity of coated sutures. Adherent S. aureus were found via SEM on all types of investigated sutures. The novel antimicrobial sutures showed significantly less viable adhered S. aureus bacteria (up to 6.1 log) compared to Vicryl (R) Plus (0.5 log). Within 11 mu g/cm drug-containing sutures, octenidine-palmitate (OL11) showed the highest number of viable adhered S. aureus (0.5 log), similar to Vicryl (R) Plus. Chlorhexidine-laurate (CL11) showed the lowest number of S. aureus on sutures (1.7 log), a 1.2 log greater reduction. In addition, planktonic S. aureus in suspensions were highly inhibited by CL11 (0.9 log) represents a 0.6 log greater reduction compared to Vicryl (R) Plus (0.3 log). Conclusions Novel antimicrobial sutures can potentially limit surgical site infections caused by multiple pathogenic bacterial species. Therefore, a potential inhibition of multispecies biofilm formation is assumed. In detail tested with S. aureus, the chlorhexidine-laurate coating (CL11) best meets the medical requirements for a fast bacterial eradication. This suture coating shows the lowest survival rate of adhering as well as planktonic bacteria, a high drug release during the first-clinically most relevant-48 hours, as well as biocompatibility. Thus, CL11 coatings should be recommended for prophylactic antimicrobial sutures as an optimal surgical supplement to reduce wound infections. However, animal and clinical investigations are important to prove safety and efficacy for future applications

Integrating mechatronic thinking and multi-agent approaches

The paper introduces a concept for the combination of mechatronic unit and multi-agent system approaches. It highlights how combining both approaches to a new kind of integrated system architecture may enforce the dissemination and reusability of methods and automatisms developed for one specific system architecture to other similar ones. Additionally it will show how this integrated architecture leads to a better exchange of Automation architectures in general. Thus, all systems compliant with the described architecture may benefit from methods and automatism applicable for a broad range of systems. The suitability of this integration approach is verified by using the example of a washing machine production line

Implementation of a methodology for consideration of product quality within discrete manufacturing

The present paper deals with the questions how product quality can be influenced within product manufacturing and how production control can be optimized for increasing product quality. It focuses on discrete manufacturing processes and presents a methodology to gather and analyze relevant influences on product quality and a multi-agent architecture for flexible and quality focused production control. It will be shown how both approaches can be implemented to achieve a flexible, adaptable and quality focused production process control

Methodology for consideration of system quality within manufacturing

The present paper deals with the question how system quality can be influenced within its engineering and manufacturing. It focuses on discrete manufacturing by system of systems and presents a methodology to gather and analyze relevant influences on system quality within a MPFQ-model. The model is based on four main elements: Materials, Processes, Functions and Quality. The paper brings together functional design/thinking in system of systems engineering with system engineering and manufacturing design. It will show the basic architecture behind the MPFQ model and how quality influences can be methodically detected. Afterwards the application of the MPFQ-model will be shown and benefits will be evaluated

Adaptation of functional inspection test plan in a production line using a multi-agent system

The multi-agent systems technology is a proper approach to implement distributed manufacturing systems exhibiting adaptation and flexibility. This paper proposes a multi-agent based solution for the adaptation of the functional test plan in a production line producing washing machines, aiming to increase the process productivity and product quality. The global adaptation mechanism is embedded on the multiagent system infrastructure, allowing the optimized selection of tests based on the correlation of the quality data gathered along the production line. The proposed approach was developed and installed on a real production line producing washing machines under the European Seventh Framework Programme GRACE project

The RNA-binding protein repertoire of embryonic stem cells

RNA-binding proteins (RBPs) have essential roles in RNA-mediated gene regulation, and yet annotation of RBPs is limited mainly to those with known RNA-binding domains. To systematically identify the RBPs of embryonic stem cells (ESCs), we here employ interactome capture, which combines UV cross-linking of RBP to RNA in living cells, oligo(dT) capture and MS. From mouse ESCs (mESCs), we have defined 555 proteins constituting the mESC mRNA interactome, including 283 proteins not previously annotated as RBPs. Of these, 68 new RBP candidates are highly expressed in ESCs compared to differentiated cells, implicating a role in stem-cell physiology. Two well-known E3 ubiquitin ligases, Trim25 (also called Efp) and Trim71 (also called Lin41), are validated as RBPs, revealing a potential link between RNA biology and protein-modification pathways. Our study confirms and expands the atlas of RBPs, providing a useful resource for the study of the RNA-RBP network in stem cells. © 2013 Nature America, Inc. All rights reserved.11771801sciescopu